Buffers maintain their pH when small amounts of strong acids or bases are added. In biology, buffer solutions are used to provide a constant pH environment for biochemical processes. The pH often changes the environments of tissues and cells and can alter the activity of biomolecules. Buffers maintain the correct pH for proteins and oligonucleotides, and are especially important for techniques like electrophoresis.
Commonly used buffers in life science research share some of these characteristics:
- A pKa value between 6.0 and 8.0
- High water solubility
- Minimal changes due to temperature and concentration
- Limited effects due to ionic or salt composition of the solution
Browse our comprehensive selection of buffers including common buffers tris(hydroxymethyl)aminomethane (tris) and 4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid (HEPES).
TBE 10X BUFFER (1L) (fr)
TBE buffer maintains the structural integrity of nucleic acids and more suitable for their size analysis. TBE has a greater buffering capacity and will give sharper resolution. TBE is better suited for high-voltage (>150 V) electrophoresis because of its higher buffering capacity.