Cell Culture Reagents

59 Results Found

Lamb Serum

This serum is derived from whole blood of healthy young lambs.

Human Serum, Pooled (HCV, HIV, HBsAG Free)

This is pooled human serum obtained from normal, clotted blood.

Human Type Ab Serum (HCV, HIV, HbsAG Free)

This serum is collected from Type AB human blood.

Human Type Ab Serum From Male Donors

This serum is collected from Type AB human blood.

Human Type Ab Serum (HCV, HIV, HbsAG Free), Heat Inactivated

This serum is collected from Type AB human blood.

Neutral Red Solution (100 mL)

1:300 Solution. Used as a red nuclear counterstain in various cytochemical procedures.

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Trypan Blue, 0.4% Solution In PBS

Trypan Blue is a blue acid dye with a strong affinity for cellulose containing substrates such as cotton; less affinity for proteinaceous materials. Trypan blue solution may be used in trypan blue based cytotoxitiy and proliferation assays. Trypan Blue is used as a vital dye which is especially important because it is taken up by the reticuloendothelial system. Clark describes assays for the study of teratogenic action of trypan blue on embryonic tissues using Davis and Sauter's fluorescence method and for the staining of collagen, including very fine fibrils, muscle and cornified epithelium using the Van Gieson method. Trypan blue is also recommended for use in dye exclusion procedures for viable cell counting. Non-viable cells will up-take trypan blue at a faster rate than viable cells.

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Water For Cell Culture

For use in the preparation of cell culture media, and cell suspension and washing solutions.

}{3 AT (POWDER) UN3077-III (9)

Bulk powder, easily weighed to make custom batch sizes.

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}{ 5-FOA (POWDER)

Bulk powder, easily weighed to make custom batch sizes.

Soy Hydrolysate Enzymatic

Soy Hydrolysate Enzymatic

Yeast Hydrolysate Enzymatic

Yeast Hydrolysate Enzymatic

Poly-L-Lysine Hydrobromide

Poly-L-Lysine Hydrobromide

Endothelial Cell Growth Supplement

(ECGS)
From Bovine Hypothalamus
Lyophilized from sodium chloride buffer containing streptomycin sulfate
Activity: promotes the growth of Fetal Bovine Heart Endothelial Cells (FBHEC) in 10% newborn calf serum when reconstituted at a concentration of 50-150 μg/ml serum-free medium.
It contains several growth factors (e.g. FGF, ECGF-α) and supports the growth of vascular endothelial cells from human and other mammalian species. Also, it may be used for the cloning and proliferation of hybridomas and human keratinocytes.
Each lot is sterile filtered and tested for bacteria, fungi, and mycoplasma contamination. Some cells may require supplementation with 1-100 μg/ml heparin.

Epidermal Growth Factor 95%

Epidermal growth factor or EGF is a growth factor isolated from mouse submaxillary glands that stimulates cell growth, proliferation, and differentiation by binding to its receptor EGFR. EGF is mitogenic for a variety of epidermal and epithelial cells, including fibroblasts, glial cells, mammary epithelial cells, vascular and corneal endothelial cells, bovine granulosa, rabbit chondrocytes, HeLa and SV40-3T3 cells.

Hemoglobin

Hemoglobin is the major component of erythrocytes, and is responsible for the red color. Its normal concentration in erythrocytes is approximately 34%. It is the most important respiratory protein of vertebrates by virtue of its ability to transport oxygen to tissues and carries carbon dioxide away from the tissues. Anomalous globins in which various amino acids have been substitued with others, or in which certain amino acids are missing entirely from the normal sequence, comprise 153 abnormal hemoglobin configurations. Sickle cell anemia is one such configuration. In sickle cell hemoglobin, a valine residue has replaced the glutamic acid residue at position 6 in the b-chain of normal hemoglobin; the a-chain is normal.

1M HEPES Buffer

HEPES (N-2-hydroxyethylpiperazine-N-2-ethane sulfonic acid) is a general purpose zwitterionic organic chemical buffering agent which does not bind magnesium, calcium, manganese(II) or copper (II) ions. Buffer strength for cell culture applications is usually in the range of 10 to 25 mM. Studies have indicated that 20 mM HEPES is the most satisfactory concentration of the buffer when both Hanks' and Earle's solutions are used.

HEPES Buffer

Powdered
Cell Culture Grade
Storage temperature: 15-30°C