Culture Media

276 Results Found

TRANSFORM & GROW BACTERIAL TRA

- No Heat Shock Required - Transform-It™ Solution is Ready-to-Use - Transform Plasmids, Ligations, and Cosmids - Store Competent Cells 10 Weeks or Longer - More Reliable than “Do-it-Yourself” Methods - Easy Procedure with Minimal Hands-On Time

EZ-YEAST TRANSFORMATION KIT

- Simple and fast with minimal hands-on time - No need to make cells competent prior to transformation - High throughput: can be easily adapted to 96-well format - Ideal for simultaneous transformation of library and bait vectors into yeast two-hybrid reporter strains - Transformation efficiency up to 1 x 103 colonies per μg DNA

CIRCLEGROW (STERILE SOLUTION)

Superior Plasmid Yield Through Enhanced Cell Growth Perfect for overnight or weekend cultures, CIRCLEGROW™ Medium is a super-rich bacterial growth medium that produces up to three times more plasmid DNA than traditional growth medium. CIRCLEGROW™ Medium consists of a protein and amino acid source, biological buffer, and a complex carbon source that gets metabolized at a much slower rate than glucose, thus prolonging the cell cycle and preventing cell death during the log phase of growth. Cells grown with CIRCLEGROW™® Medium remain healthy for more than two days and continue to divide slowly after the log phase of the growth has been completed. Reduce Hands-On Work and Preparation Time CIRCLEGROW™ cultures do not need to be constantly monitored by O.D. readings due to the incremental post-log cell growth. Obtain excellent yields of plasmid DNA from smaller culture volumes, and confidently leave cultures overnight or over the weekend. CIRCLEGROW™ capsules are the most convenient form of this unique growth media. Pre-mixed CIRCLEGROW™ is also available in bulk powder, single-use pouches, capsules and sterile liquid.

ALKALI-CATION YEAST TRANSFORMA

The Alkali-Cation™ Yeast Transformation Kit provides the reagents needed to perform an easy and quick transformation of yeast, while obtaining a large number of transformants. Its ease of use, robustness, and scalability make this the method of choice for the high-efficiency transformation needed in two hybrid screens. Host cells are made competent by treatment with a lithium/cesium acetate solution. Plasmid DNA is added to the competent cells along with carefully prepared carrier DNA that has been optimized to provide high transformation effi ciencies. The Alkali-Cation™ Yeast Transformation Kit has also been successfully used to transform linear DNA into P. pastoris for protein expression studies.

CSM-HIS-LEU-TRP W/40 MG/L ADE

Bulk powder, easily weighed to make custom batch sizes.

CSM-LEU W/40 MG/L ADE (POWDER)

Bulk powder, easily weighed to make custom batch sizes.

CSM-LEU-URA (POWDER)

Bulk powder, easily weighed to make custom batch sizes.

CSM-TRP W/20 MG/L ADE (POWDER)

Bulk powder, easily weighed to make custom batch sizes.

CSM-URA W/20 MG/L ADE (POWDER)

Bulk powder, easily weighed to make custom batch sizes.

CSM-URA W/40 MG/L ADE (POWDER)

Bulk powder, easily weighed to make custom batch sizes.

DOB 2% GALACTOSE (POWDER)

Bulk powder, easily weighed to make custom batch sizes.

DOBA W/ 2% GALACTOSE (POWDER)

Bulk powder, easily weighed to make custom batch sizes.

EMM (PACK)(POUCHES)

Pre-measured, sealed pouches, for 0.5 L or 1 L batch sizes, simply tear open the pouch and add to water.

EMM NO NITROGEN (NO NH[4]CL, G

Bulk powder, easily weighed to make custom batch sizes.

HL5 MEDIUM (POWDER)

Bulk powder, easily weighed to make custom batch sizes.

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LB-AGAR, LENNOX (LARGE CAPSULE

Encapsulated Media: the Ultimate in Convenience. Simply count out the appropriate number of capsules and drop them in water! |- No weighing. No dust. No cleanup. No smell. - Simply drop capsules in water and autoclave - Unbeatable selection of formulations - Also available in other pre-measured formats

LB-AGAR, LENNOX (POWDER)

Bulk powder, easily weighed to make custom batch sizes.

M9 MINIMAL MEDIUM SALTS (PART

Bulk powder, easily weighed to make custom batch sizes.

NZY-HARVARD BROTH (POWDER)

Bulk powder, easily weighed to make custom batch sizes.

1X RPMI Without L-Glutamine, L-Cysteine, L-Cystine, and L-Methionine

RPMI 1640 Medium was originally developed by Moore and his colleagues at Roswell Park Memorial Institute (RPMI). It was based on the RPMI 1630 line of media which utilized a bicarbonate buffering system and alterations in the amounts of amino acids and vitamins. A Dutch Modification of RPMI 1640 is also available through MP. The Dutch Modification contains 6400 mg/L sodium chloride instead of 6000 mg/L; contains 1000 mg/L sodium bicarbonate instead of 2000 mg/L; and contains 20 mM HEPES.